Recombinant Clusterin antibody

Catalog No. ABIN7865336

The Mouse Monoclonal anti-Clusterin antibody (Clone r7D1) (ABIN7865336) specifically detects Clusterin in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Clusterin antibody (ABIN7865336)

Target: Clusterin (CLU)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: R7D1

Product Details anti-Clusterin Antibody

Expression System: CHO Cells

Purpose: Recombinant Clusterin / Apolipoprotein J (APO-J) (Marker of Anaplastic Large Cell Lymphoma) Antibody

Specificity: Clusterin, also known as apolipoprotein J (ApoJ), complement lysis inhibitor (CLI), gp80, glycoprotein III, sulfated glycoprotein 2 (SGP2), SP40-40, testosterone-repressed prostate message 2 (TRPM2), and T64, is a secretory, heterodimeric glycoprotein of approximately 80 kD composed of two disulfide-linked subunits, alpha (residues 228,Äi449) and beta (residues 23,Äi227). It is synthesized as a 449-amino acid polypeptide and post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Clusterin interacts with a broad range of molecules and plays a role in diverse biological processes, including immune regulation, lipid transport, sperm maturation, complement cascade regulation, membrane recycling, cell adhesion, morphological transformation, tissue remodeling, and cell-cell interactions. It also influences ≤-amyloid structure and neuritic toxicity in vivo, suggesting a potential role in Alzheimer,Ä111s disease pathogenesis. Notably, Clusterin overexpression is more common in the late stages of mammary tumor progression.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant protein corresponding to a portion of the alpha subunit of the human clusterin molecule.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background:

Aging associated protein 4, Apolipoprotein J, CLU1, CLU2, Clusterin alpha / beta chain, Complement associated protein SP40, Complement cytolysis inhibitor a chain, NA1/NA2, D14Ucla3, Dimeric acid glycoprotein, GP80, Ku70-binding protein 1 (KUB1), Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B)

Human pancreas, tonsil or cervix.

Molecular Weight: 70kDa (precursor), 36-39kDa (alpha), 34-36kDa (beta)

Gene ID: 1191, 436657

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling