Recombinant FOLR1 antibody

Catalog No. ABIN7857792

The Rabbit Monoclonal anti-FOLR1 antibody is suitable to detect FOLR1 in samples from Human, Mouse, Rat, Guinea Pig and Hamster. It has been validated for WB and IHC.

Quick Overview for Recombinant FOLR1 antibody (ABIN7857792)

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FOLR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: FOLR1-12910R

Product Details anti-FOLR1 Antibody

Expression System: CHO Cells

Purpose: Recombinant Folate Receptor Alpha (FRalpha) / FOLR1 Antibody

Specificity: Folate receptor alpha (FR±) is a membrane-bound protein that facilitates high-affinity folate transport, essential for cell metabolism, DNA synthesis, and repair, particularly in rapidly dividing cancer cells with increased folate demands. This receptor, encoded by the FOLR1 gene, employs a potocytosis mechanism that leads to both membranous and cytoplasmic localization and is selectively overexpressed in certain epithelial malignancies while remaining highly restricted in normal tissues. FR± expression correlates with tumor stage and grade, suggesting a role in tumor progression by enhancing folate uptake or generating regulatory signals. Its regulation is influenced by extracellular folate levels, homocysteine accumulation, hormones, transcription factors, and genetic mutations. Members of the folate receptor family share highly conserved sequences in the open reading frames but differ in amino acids in the 5' untranslated regions and as a consequence can differ in function and tissue expression. Given the selective overexpression of FR± in tumors and its association with aggressive cancer phenotypes, FR± is a valuable marker for studying tumor biology and progression, particularly in epithelial cancers.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment of human FOLR1 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FOLR1

Target: FOLR1 (Folate Receptor 1 (Adult) (FOLR1))

Alternative Name: Folate Receptor Alpha

Background:

DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E(WS2E), WS4, Waardenburg syndrome type 4C (WS4C)

Human kidney, thymus, placenta or lung. Human Brain, Mouse Brain, Rat Brain, Hamster Brain or Guinea pig Brain

Molecular Weight: 29kDa

Gene ID: 2348

UniProt: P15328

Pathways: Dicarboxylic Acid Transport