Recombinant FAS antibody

Catalog No. ABIN7865082

The Rabbit Monoclonal anti-FAS antibody (Clone FAS-9322R) (ABIN7865082) specifically detects FAS in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant FAS antibody (ABIN7865082)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This FAS antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: FAS-9322R

Product Details anti-FAS Antibody

Expression System: CHO Cells

Purpose: Recombinant CD95 / FAS / TNFRSF6 Antibody

Specificity: MAb FAS/9322R specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45 kDa, containing 8 kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant human CD95 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for FAS

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Alternative Name: CD95

Background:

ALPS1A, Apoptosis APO1 antigen 1, Apoptosis-mediating surface antigen FAS, APT1, Delta Fas, AMA, Fas cell surface death receptor, FAS1, FASL receptor, FASLG receptor, FASTM, Surface antigen APO1, Tumor necrosis factor receptor superfamily member 6 (TNFRSF6)

MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).

Molecular Weight: 38-50kDa

Gene ID: 355, 244139

UniProt: P25445

Pathways: p53 Signaling, Apoptosis, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity