Recombinant Nucleolin antibody

Catalog No. ABIN7863187

The Rabbit Monoclonal anti-Nucleolin antibody is suitable to detect Nucleolin in samples from Human. It has been validated for WB, IHC, IF and FACS.

Quick Overview for Recombinant Nucleolin antibody (ABIN7863187)

Target: Nucleolin (NCL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: NCL-7014R

Product Details anti-Nucleolin Antibody

Expression System: HEK-293 Cells

Purpose: Recombinant Nucleolin (Marker of Human Cells) Antibody

Specificity: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant full-length human NCL protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background:

NCL, Nucl, Nucleolin, Protein C23

All human cells. Human testis, ovary or Hodgkin s lymphoma.

Molecular Weight: 76kDa

Gene ID: 4691, 79110

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization