Recombinant MYOD1 antibody (AA 1-100)

Catalog No. ABIN7864359

This Rabbit Monoclonal antibody specifically detects MYOD1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant MYOD1 antibody (AA 1-100) (ABIN7864359)

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MYOD1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MYOD1-9405R

Product Details anti-MYOD1 Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant MyoD1 (Rhabdomyosarcoma Marker) Antibody

Specificity: MyoD1, one of the MyoD family of myogenic helix-loop-helix transcription factors, combined with myogenin, plays a role in coordinating the myogenic differentiation pathway from the determination of mesodermal precursors into myoblasts, the differentiation of myoblasts into myotubes, and finally the maturation of myotubes into skeletal myofibers. Normal mature skeletal muscle does not express MyoD1 protein. MyoD1 is expressed in myoblasts before differentiation while myogenin has post-differentiation functions. Anti-MyoD1 immunostaining identifies cells committed to myogenesis in their earliest phase, thus, it is a better biomarker for less differentiated Rhabdomyosarcomas (RMS). RMS are the most frequent malignant soft tissue neoplasms of childhood. While better differentiated RMS have cross-striations or rhabdomyoblasts that allow for a confident morphologic diagnosis, less differentiated RMS resemble other small blue round-cell tumors. Studies suggest, anti-MyoD1 may be used together with anti-Myogenin and anti-Desmin as a panel of markers since any RMS is virtually never negative for all three markers simultaneously.

Purification: 200ug/ml of Ab purified by Protein A Column.

Immunogen: Recombinant fragment (around aa1-100) of human MyoD1 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for MYOD1

Target: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Alternative Name: MyoD1

Background:

bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM

Human fetal skeletal muscle or rhabdomyosarcoma.

Molecular Weight: 45kDa

Gene ID: 4654, 181768

UniProt: P15172

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development