Recombinant CD20 antibody (AA 200-297)

Catalog No. ABIN7857938

This Mouse Monoclonal antibody specifically detects CD20 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD20 antibody (AA 200-297) (ABIN7857938)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RMS4A1-8044

Product Details anti-CD20 Antibody

Binding Specificity: AA 200-297

Purpose: Recombinant CD20 / MS4A1 (B-Cell Marker) Antibody

Specificity: Recognizes a protein of 30-33 kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Purification: 200ug/ml of Ab purified by Protein A Column.

Immunogen: Recombinant fragment (around aa200-297) of human MS4A1 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Alternative Name: CD20

Background:

APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, MS4A1, MS4A2

and U266|Daudi|or human lymphocytes. Human lymph node or tonsil.|Raji

Molecular Weight: 33-37kDa

Gene ID: 931, 712553