RENT1/UPF1 antibody

Catalog No. ABIN7865540

This Mouse Monoclonal antibody specifically detects RENT1/UPF1 in ELISA. It exhibits reactivity toward Human.

Quick Overview for RENT1/UPF1 antibody (ABIN7865540)

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RENT1/UPF1 antibody is un-conjugated

Application: ELISA

Clone: NY1-1-1B6

Product Details anti-RENT1/UPF1 Antibody

Purpose: RENT1 / hUPF1 Antibody

Specificity: In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability. Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP and directly interacts with Rent2, which is a mammalian homolog of Upf2p. Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes. Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent1 and Rent2 localize primarily in the cytoplasm. Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A.

Immunogen: Recombinant human UPF1 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for RENT1/UPF1

Target: RENT1/UPF1 (UPF1) (UPF1 Regulator of Nonsense Transcripts Homolog (UPF1))

Alternative Name: RENT1

Background:

KIAA0221, RENT1, UPF1, UTF, smg-2, pNORF1

HeLa cells.

Molecular Weight: 124kDa

Gene ID: 5976

UniProt: Q92900

Pathways: SARS-CoV-2 Protein Interactome