Recombinant E2F4 antibody (AA 1-100)

Catalog No. ABIN7864679

This Rabbit Monoclonal antibody specifically detects E2F4 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant E2F4 antibody (AA 1-100) (ABIN7864679)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: E2F4-8811R

Product Details anti-E2F4 Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant E2F4 (Transcription Factor) Antibody

Specificity: The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-100) of human E2F4 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background:

E2F transcription factor 4, E2F transcription factor 4 p107/p130 binding, E2F-4, p107/p130 binding protein

Brain|Found in all tissue examined including heart|kidney and pancreas.|liver|lung|Placenta|skeletal muscle

Molecular Weight: 60kDa

Gene ID: 1874, 108371

UniProt: Q16254

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size