Recombinant Iba1 antibody (AA 1-146)

Catalog No. ABIN7858265

This Rabbit Monoclonal antibody specifically detects Iba1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Iba1 antibody (AA 1-146) (ABIN7858265)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: AIF1-8970R

Product Details anti-Iba1 Antibody

Binding Specificity: AA 1-146

Purpose: Recombinant AIF1 / Iba1 (Microglia Marker) Antibody

Specificity: AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background:

Allograft inflammatory factor 1 (AIF1), balloon angioplasty responsive transcription (BART1), IBA1, Interferon responsive transcript 1, Ionized calcium-binding adapter molecule 1, IRT1, Microglia response factor (MRF1)

Human lymph node|tonsil or kidney. Human Brain, Human Liver, Human Spleen and Raw

Molecular Weight: 17kDa

Gene ID: 199, 76364

UniProt: P55008

Pathways: Smooth Muscle Cell Migration