IL-18 antibody (AA 1-193)

Catalog No. ABIN7858336

The Mouse Monoclonal anti-IL-18 antibody (Clone IL18-4623) (ABIN7858336) specifically detects IL-18 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for IL-18 antibody (AA 1-193) (ABIN7858336)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL-18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: IL18-4623

Product Details anti-IL-18 Antibody

Binding Specificity: AA 1-193

Purpose: IL-18 (Interleukin-6) / Interferon beta-2 (Hybridoma Growth Factor) Antibody

Specificity: Four structurally related IL-1 receptor ligands have been described. These include three agonists designated IL-1±, IL-1≤ and IL-1≥/IL-18 and a specific receptor antagonist, IL-1R±. IL-1± and IL-1≤ play critical roles in the regulation of the immune response and inflammation, serving as activators of T and B lymphocytes and NK (natural killer) cells. IL-18 (also referred to as IL-1≥) has been shown to augment the secretion of IFN-≥ from T lymphocytes and increase NK cell activity in spleen cells. IL-18 exhibits 19 % and 12 % identity with IL-1± and IL-1≤ respectively over the 12 ≤-strands of the ≤-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1≤ pro-domain which must be cleaved by the serine protease ICE for optimal secretion and biological activity. Originally described as IGIF (IFN-≥-inducing factor), IL-18 is induced by mouse liver subsequent to challenge with lipopolysaccharide (LPS).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-193) of human IL-18 protein (exact sequence is proprietary)

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL-18

Background:

Iboctadekin, IFN gamma inducing factor, IGIF, IL1 gamma, IL18, IL18 protein, IL1F4, IL1g, IL1gamma, Interleukin 18, Interleukin-1 gamma, MGC12320

A459 cells, Human heart or adrenal gland. Stimulated peritoneal macrophages.

Molecular Weight: 18kDa

Gene ID: 3606, 83077

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome