Caspase 3 antibody (AA 1-200)

Catalog No. ABIN7865116

This Mouse Monoclonal antibody specifically detects Caspase 3 in IHC. It exhibits reactivity toward Human.

Quick Overview for Caspase 3 antibody (AA 1-200) (ABIN7865116)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CASP3-8446

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 1-200

Purpose: Caspase 3 (Pro & Active Form) Antibody

Specificity: This MAb recognizes a protein of 32 kDa, identified as Caspase 3. It is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp 175-Ser 176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp 28-Ser 29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 sub- units. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at a Asp 216-Gly 217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human CASP3 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase 3

Background:

APOPAIN, Apoptosis related cysteine peptidase, CASP3, Caspase3, CPP32, CPP32B, cysteine protease CPP32, PARP cleavage protease, Procaspase3, SREBP cleavage activity 1 (SCA1), Yama

Heart|HL60 or Jurkat cells. Human liver|liver or kidney.|spleen

Molecular Weight: 32kDa

Gene ID: 836, 141125

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation