IRS1 antibody (AA 1-200)

Catalog No. ABIN7864809

The Mouse Monoclonal anti-IRS1 antibody (Clone IRS1-7571) (ABIN7864809) specifically detects IRS1 in IHC. The antibody is reactive with Human samples.

Quick Overview for IRS1 antibody (AA 1-200) (ABIN7864809)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: IRS1-7571

Product Details anti-IRS1 Antibody

Binding Specificity: AA 1-200

Purpose: IRS1 (Insulin receptor substrate 1) Antibody

Specificity: The Insulin receptor substrate-1 (IRS-1), a protein major substrate of the Insulin receptor, is phosphorylated in response to stimulation of cells by Insulin, Insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An Insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the Insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase IIcatalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase IIcatalyzed phosphorylation of IRS-1 may be a component of the intracellular Insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the Insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of Insulin sensitivity by interrupting the association between the Insulin receptor and IRS-1.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human IRS1 protein (exact sequence is proprietary)

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1

Background:

HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1, IRS1_HUMAN, OTTHUMP00000164234

adrenal gland or lung.|Human breast carcinoma

Molecular Weight: 170-185kDa

Gene ID: 3667, 445868

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process