TFAM antibody (AA 1-200)

Catalog No. ABIN7858724

This Mouse Monoclonal antibody specifically detects TFAM in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for TFAM antibody (AA 1-200) (ABIN7858724)

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: TFAM-9713

Product Details anti-TFAM Antibody

Binding Specificity: AA 1-200

Purpose: TFAM / TCF6 (Transcription factor A, mitochondrial) / mtTFA Antibody

Specificity: MtTFA (mitochondrial transcription factor A), also known as mtTF1, TFAM, TCF6 (for transcription factor 6-like1), TCF6L2 and tsHMG, is a nuclear-encoded gene product that is imported into the mitochondria. mtTFA is required for many aspects of mitochondrial biogenesis including the replication and transcription of mitochondrial DNA (mtDNA). In mouse, testis-specific mtTFA is missing the mitochondria targeting sequence and is present in the nucleus rather than the mitochondria. This form of mtTFA is located primarily in the nuclei of elongated spermatids and may be involved in the regulation of gene expression of the haploid male genome. During mouse and human spermatogenesis there is a reduction of mtTFA protein levels and a reduction in mtDNA copy number. These features may provide one of the mechanisms by which paternal mtDNA transmission is prevented. mtTFA has been associated with mitochondrial disorder in humans characterized by ocular myopathy, exercise intolerance and muscle wasting.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human TFAM protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: TFAM

Background:

TCF6, MTTF1, MTTFA, TCF6L1, TCF6L2, TCF6L3, MTDPS15

HEK293T

Molecular Weight: 25kDa

Gene ID: 7019, 642966

UniProt: Q00059

Pathways: Chromatin Binding