Recombinant CD25 antibody (AA 1-250)

Catalog No. ABIN7858773

The Rabbit Monoclonal anti-CD25 antibody is suitable to detect CD25 in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant CD25 antibody (AA 1-250) (ABIN7858773)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: IL2RA-9378R

Product Details anti-CD25 Antibody

Binding Specificity: AA 1-250

Purpose: Recombinant CD25 / IL2RA (Activated Lymphocyte Marker) Antibody

Specificity: CD25 is the alpha chain of the IL-2 receptor. It is a Type I transmembrane protein present on activated T-cells, activated B-cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. It is expressed in most B-cell neoplasms, some Acute Non-lymphocytic Leukemias, and Neuroblastomas. Expression of CD25 is a reliable diagnostic tool for distinguishing neoplastic mast-cell aggregates from reactive proliferations, and has, therefore, recently become a minor criterion for the diagnosis of Systemic Mastocytosis (SM). Anti-CD25 antibodies have also been useful in identifying mast cells in skin biopsies in the setting of Urticaria Pigmentosa, which is predictive of Systemic Mastocytosis. Quantitation of regulatory T-cells (Treg) in the setting of hepatocellular carcinoma has been used as an independent predictive factor for tumor recurrence after hepatic resection for HCC. Also, the percentage of tumor-infiltrating CD25+FOXP3+regulatory T-cells among tumor cells, inside tumor parenchyma and at its periphery are significantly higher in recurrent Cutaneous Melanoma than in Non-recurrent Melanoma.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-250) of human IL2RA protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background:

IL2 Receptor alpha, IL2R alpha chain, IL2R, IL2R subunit alpha IL2RA, Interleukin-2 receptor subunit alpha, Ly43, p55, p55 chain, T Cell Growth Factor Receptor, TAC antigen, TCGFR

Human tonsil, small bowel, spleen, mastocytosis, or Hodgkin,Ä111s lymphoma.

Molecular Weight: 31kDa

Gene ID: 3559, 231367

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation