Recombinant BCL6 antibody (AA 1-400)

Catalog No. ABIN7858846

The Rabbit Monoclonal anti-BCL6 antibody (Clone BCL6-9222R) (ABIN7858846) specifically detects BCL6 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant BCL6 antibody (AA 1-400) (ABIN7858846)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: BCL6-9222R

Product Details anti-BCL6 Antibody

Binding Specificity: AA 1-400

Purpose: Recombinant Bcl-6 (Follicular Lymphoma Marker) Antibody

Specificity: Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ( L&H ) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (aa1-400) of human BCL6 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: Bcl-6

Background:

B-cell lymphoma 5 protein, B-Cell Lymphoma 6 Protein, BCL5, BCL6, BCL6A, cys his2 zinc finger transcription factor, Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3), Zinc finger and BTB domain-containing protein 27 (ZBTB27), Zinc Finger Protein 51 (ZNF51), zinc finger transcription factor BCL6S

Raji or Ramos cells. Human tonsil or Hodgkin s lymphoma.

Molecular Weight: 95kDa

Gene ID: 604, 478588

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus