Nanog antibody (AA 100-250)

Catalog No. ABIN7858967

This Mouse Monoclonal antibody specifically detects Nanog in IHC. It exhibits reactivity toward Human.

Quick Overview for Nanog antibody (AA 100-250) (ABIN7858967)

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nanog antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: NANOG-9704

Product Details anti-Nanog Antibody

Binding Specificity: AA 100-250

Purpose: Homeobox protein NANOG (Cancer Stem Cell Marker) Antibody

Specificity: The homeobox protein Nanog is a transcription factor crucial for maintaining the pluripotency of embryonic stem cells (ESCs) by inhibiting cell differentiation factors. In humans, the NANOG gene encodes this protein. Nanog operates alongside other factors such as Oct-4 and SOX2 to define ESC identity. It is also highly expressed in cancer stem cells, suggesting a potential role as an oncogene in promoting cancer development. Elevated levels of Nanog are linked to poor prognosis in cancer patients. Nanog shows strong and specific expression in carcinoma in situ (CIS), embryonal carcinomas, and seminomas but are absent in teratomas and yolk sac tumors. Research indicates that human embryonic stem cell-related genes, including OCT4, NANOG, STELLAR, and GDF3, are expressed in seminomas and breast carcinomas. Nanog positivity is notably associated with high-grade ovarian serous carcinoma but is not observed in benign, borderline, or low-grade serous lesions. One study highlights the cellular shuttling of Nanog and its increased stromal presence during cervical cancer progression. Furthermore, Nanog overexpression has been linked to factors such as tumor differentiation, lymph node metastasis, and tumor size, with studies suggesting its predictive value for reduced overall survival (OS) and disease-free survival (DFS) in lung cancer.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa100-250) of human NANOG protein (exact sequence is proprietary)

Isotype: IgG2b, lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Nanog

Target: Nanog (NANOG) (Nanog Homeobox (NANOG))

Alternative Name: Homeobox protein NANOG

Background:

homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48

Human testicular carcinoma.

Molecular Weight: 40kDa

Gene ID: 79923

UniProt: Q9H9S0

Pathways: Stem Cell Maintenance