Clusterin antibody (AA 185-287)

Catalog No. ABIN7862637

The Mouse Monoclonal anti-Clusterin antibody is suitable to detect Clusterin in samples from Human. It has been validated for IHC.

Quick Overview for Clusterin antibody (AA 185-287) (ABIN7862637)

Target: Clusterin (CLU)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CLU-4730

Product Details anti-Clusterin Antibody

Binding Specificity: AA 185-287

Purpose: Clusterin / Apolipoprotein J (APO-J) Antibody

Specificity: Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, °™ and æ, are associated through disulfide bonds. The æ subunit (also called ApoJ°™) corresponds to residues 23-227. The °™ subunit (also called ApoJ æ) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences æ-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa185-287) of human NPM1 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: Clusterin

Background:

Aging associated protein 4, Apolipoprotein J, CLU1, CLU2, Clusterin alpha / beta chain, Complement associated protein SP40, Complement cytolysis inhibitor a chain, NA1/NA2, D14Ucla3, Dimeric acid glycoprotein, GP80, Ku70-binding protein 1 (KUB1), Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B)

Human pancreas, tonsil or cervix.

Molecular Weight: 70kDa (precursor), 36-39kDa (°™), 34-36kDa (•æ)

Gene ID: 1191, 436657

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling