OLIG2 antibody (AA 200-313)

Catalog No. ABIN7864430

The Mouse Monoclonal anti-OLIG2 antibody (Clone rOLIG2-8884) (ABIN7864430) specifically detects OLIG2 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for OLIG2 antibody (AA 200-313) (ABIN7864430)

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ROLIG2-8884

Product Details anti-OLIG2 Antibody

Binding Specificity: AA 200-313

Purpose: OLIG2 (Marker of Glial Brain Tumors) Antibody

Specificity: Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human OLIG2 protein (around aa 200-313) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: OLIG2

Background:

Basic helix loop helix protein class B1 (bHLHB1), basic helix-loop-helix protein 19 (bHLHe19), OLIG2, Oligodendrocyte lineage transcription factor 2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2 (PRKCBP2), RACK17

THP-1 cells. Human brain or astrocytoma.

Molecular Weight: 30-40kDa

Gene ID: 10215, 176977