BRCA2 antibody (AA 200-500)

Catalog No. ABIN7864856

This Mouse Monoclonal antibody specifically detects BRCA2 in IHC. It exhibits reactivity toward Human.

Quick Overview for BRCA2 antibody (AA 200-500) (ABIN7864856)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: BRCA2-2158

Product Details anti-BRCA2 Antibody

Binding Specificity: AA 200-500

Purpose: BRCA2 (Breast Marker) Antibody

Specificity: In 1990, a breast cancer susceptibility gene designated BRCA1 was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45 % of families with high incidence of breast cancer and at least 80 % of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2 (breast cancer 2, early onset), located on chromosome 13q13.1, also confers a high incidence of breast cancer but, unlike BRCA1, does not confer a substantially elevated risk of ovarian cancer. Both BRCA1 and BRCA2 play a role in the maintainance of genome stability, particularly in the homologous recombination pathway for double-strand DNA repair. BRCA2 is regarded as a tumor suppressor gene, tumors with BRCA2 mutations exhibit loss of heterozygosity (LOH) of the wildtype allele. The protein encoded by the BRCA2 gene contains multiple copies of a 70 amino acid motif called the BRC motif. These motifs effect binding to the Rad51 recombinase, which operates in DNA repair.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 200-500) of human BRCA2 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2

Background:

BRCA1/BRCA2 containing complex subunit 2, BRCA2, DNA repair associated, BRCC2, Breast and ovarian cancer susceptibility gene early onset, breast and ovarian cancer susceptibility protein 2, Breast cancer 2 early onset, Breast Cancer 2 tumor suppressor, BROVCA2, FACD, FAD1, FANCB, FANCD, FANCD1, Fanconi anemia complementation group D1, GLM3, mutant BRCA2, PNCA2, XRCC11

A431 or HeLa cells. Human breast|lung|ovary or spleen.|thymus

Molecular Weight: 390kDa

Gene ID: 675, 34012

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location