Recombinant Ki-67 antibody (AA 2293-2478)

Catalog No. ABIN7863141

The Rabbit Monoclonal anti-Ki-67 antibody (Clone MKI67-4945R) (ABIN7863141) specifically detects Ki-67 in IHC and WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant Ki-67 antibody (AA 2293-2478) (ABIN7863141)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: MKI67-4945R

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 2293-2478

Purpose: Recombinant Ki-67 (Proliferating Cell Marker) Antibody

Specificity: Ki-67 antigen is a nuclear, non-histone protein that is present in all stages of the cell cycle except G0. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. A correlation has been demonstrated between Ki-67 index and the histo-pathological grade of neoplasms. Assessment of Ki-67 expression in renal and ureter tumors shows a correlation between tumor proliferation and disease progression, thus making it possible to differentiate high-risk patients. Ki-67 expression may also prove to be important for distinguishing between malignant and benign peripheral nerve sheath tumors. Ki-67 labeling index has been shown to be a prognostic marker in a number of neoplasms including grade II astrocytoma, oligodendroglioma, colon carcinoma, and breast carcinoma. In general, Ki-67 is a good marker of proliferating cell populations.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 2293-2478) of human Ki67 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background:

KI-67, Ki67, KI-67 Antigen (KIA), MKI67, Proliferation related Ki-67 antigen

Any actively proliferating cells. Human skin, tonsil or lymph node. Raji, Ramos

Molecular Weight: 345kDa and 395kDa

Gene ID: 4288, 689823

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process