PDGFB antibody (AA 27-158)

Catalog No. ABIN7863222

The Mouse Monoclonal anti-PDGFB antibody (Clone PDGFB-3071) (ABIN7863222) specifically detects PDGFB in IHC. The antibody is reactive with Human samples.

Quick Overview for PDGFB antibody (AA 27-158) (ABIN7863222)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDGFB antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PDGFB-3071

Product Details anti-PDGFB Antibody

Binding Specificity: AA 27-158

Purpose: PDGF beta (PDGFB) Antibody

Specificity: PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, AB and BB. The three isoforms bind, with different affinities, to two receptor types, °™and æ, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to °™ receptors with high affinity, whereas the B subunit can bind to both °™ and æ receptors. Evidence suggests that PDGF may function as a neurotrophic factor. Receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons. These facts suggest that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system through paracrine and autocrine routes.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human PDGFB protein (around aa 27-158) (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PDGFB

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Alternative Name: PDGF beta

Background:

Becaplermin, c sis, Oncogene SIS, PDGF Beta chain, PDGF2, Platelet derived growth factor 2, Platelet derived growth factor B chain, Platelet derived growth factor beta, Platelet derived growth factor beta polypeptide (oncogene SIS), Platelet derived growth factor beta polypeptide (simian sarcoma viral (v sis) oncogene homolog)

Human colon, kidney or lymph node.

Molecular Weight: 14kDa (monomeric B chain), 31-35kDa (dimer)

Gene ID: 5155, 1976

UniProt: P01127

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling