Recombinant Occludin antibody (AA 282-415)

Catalog No. ABIN7864075

The Rabbit Monoclonal anti-Occludin antibody (Clone OCLN-8526R) (ABIN7864075) specifically detects Occludin in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Occludin antibody (AA 282-415) (ABIN7864075)

Target: Occludin (OCLN)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: OCLN-8526R

Product Details anti-Occludin Antibody

Binding Specificity: AA 282-415

Purpose: Recombinant Occludin (OCLN) (Tight Junctions Marker) Antibody

Specificity: Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue, in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Occludin fragment around aa 282-415 (Exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: Occludin

Background:

BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin

HepG2 cells. Human kidney.

Molecular Weight: 60-82kDa

Gene ID: 100506658, 592605

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C