Recombinant SMAD4 antibody (AA 400-552)

Catalog No. ABIN7864839

The Rabbit Monoclonal anti-SMAD4 antibody (Clone SMAD4-7906R) (ABIN7864839) specifically detects SMAD4 in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant SMAD4 antibody (AA 400-552) (ABIN7864839)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: SMAD4-7906R

Product Details anti-SMAD4 Antibody

Binding Specificity: AA 400-552

Purpose: Recombinant SMAD4 / DPC4 (Pancreatic Adenocarcinoma Marker / Tumor Suppressor) Antibody

Specificity: Signaling from the ligand-activated membrane receptor serine/threonine kinases to nuclear targets is mediated by a set of evolutionarily conserved proteins known as DPC4. Upon ligand binding, the receptors of the TGF-≤ family phosphorylate SMAD proteins (SMAD1 and SMAD2). These proteins then move into the nucleus, where they activate transcription. To carry out this function, the receptor activated SMAD1 and 2 require association with the product of deleted in pancreatic carcinoma, locus 4 (DPC4), also known as SMAD4. SMAD4/DPC4 is also implicated as a tumor suppressor, since it is inactivated in more than half of pancreatic carcinomas and to a lesser extent in a variety of other cancers. The lack of SMAD4 expression is present in approximately 80 % of cases of pancreatic adenocarcinoma, but rarely in endometrial (0 %), colorectal (0 %), ovarian (3 %), lung (0 %), breast (2 %) adenocarcinomas, and malignant melanoma (4 %). SMAD4is an important marker for confirming a diagnosis of pancreatic adenocarcinoma.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa400-552) of human SMAD4 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background:

Deleted in Pancreatic Carcinoma 4 (DPC4), JIP, MAD homolog 4 (MADH4), Mothers against decapentaplegic homolog 4 (MADH4), Mothers against DPP homolog 4, MYHRS, SMAD4

HeLa, Human pancreatic ductal adenocarcinoma.

Molecular Weight: 61kDa

Gene ID: 4089, 75862

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy