Recombinant ENO2/NSE antibody (AA 416-433)

Catalog No. ABIN7864690

The Mouse Monoclonal anti-ENO2/NSE antibody is suitable to detect ENO2/NSE in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant ENO2/NSE antibody (AA 416-433) (ABIN7864690)

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENO2/NSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RENO-8857

Product Details anti-ENO2/NSE Antibody

Binding Specificity: AA 416-433

Purpose: Recombinant NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) Antibody

Specificity: This monoclonal antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. 79oΩIt it be usually employed in combination with other markers such as Synaptophysin, 79oΩChromogranin A, and Neurofilament. 79oΩ

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human NSE gamma (around aa416-433) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Alternative Name: NSE gamma

Background:

2-phospho-D-glycerate hydrolyase, ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE

cerebellum or pheochromocytoma.|Jurkat cells. Human pancreas, Heart, A549, HeLa.

Molecular Weight: ~50kDa

Gene ID: 2026, 511915