SIGLEC10 antibody (AA 500-697)

Catalog No. ABIN7864583

The Mouse Monoclonal anti-SIGLEC10 antibody is suitable to detect SIGLEC10 in samples from Human. It has been validated for IHC.

Quick Overview for SIGLEC10 antibody (AA 500-697) (ABIN7864583)

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIGLEC10 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: SIGLEC10-7581

Product Details anti-SIGLEC10 Antibody

Binding Specificity: AA 500-697

Purpose: SIGLEC10 Antibody

Specificity: Siglec-10 (sialic acid-binding Ig-like lectin 10) is a 697 amino acid protein belonging to the immunoglobulin superfamily. The N-terminal Ig-like domain of Siglec-10 contains a sialic acid-binding site and the C-terminal cytoplasmic region of Siglec-10 contains two immunoreceptor tyrosine-based inhibitor motifs (ITIMs), which are involved in the modulation of cellular responses through SH2 domains. With most expression in spleen, bone marrow and peripheral blood lymphocytes, such as monocytes, natural killer cells and eosinophils, it is likely that Siglec-10 functions as an inhibitory receptor in the immune response. Importantly, Siglec-10 mRNA is found to be highly upregulated in eosinophils at sites of inflammation in asthmatics, suggesting that Siglec-10 may be an appropriate target for new approaches of immunomodulatory therapy. There are six named isoforms of Siglec-10 that are a result of alternative splicing. All isoforms exists as single-pass transmembrane proteins, except for isoform 6, which is secreted.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa500-697) of human SIGLEC10 protein (exact sequence is proprietary)

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for SIGLEC10

Target: SIGLEC10 (Sialic Acid Binding Ig-Like Lectin 10 (SIGLEC10))

Alternative Name: SIGLEC10

Background:

mSiglec G, PRO940, sialic acid binding Ig like lectin 10 Ig like lectin 7, Sialic acid binding Immunoglobulin like lectin 10, Siglec G, siglec like gene 2, Siglec like protein 2, Siglecg, SLG2

Expressed by peripheral blood leukocytes (eosinophils|lung|monocytes and a natural killer cell subpopulation). Isoform 5 is found in lymph node|ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow|spleen a

Molecular Weight: 77kDa

Gene ID: 89790, 284813

UniProt: Q96LC7