TdT antibody (AA 52-192)

Catalog No. ABIN7862714

This Mouse Monoclonal antibody specifically detects TdT in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for TdT antibody (AA 52-192) (ABIN7862714)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TdT antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Clone: DNTT-1453

Product Details anti-TdT Antibody

Binding Specificity: AA 52-192

Purpose: TdT / DNA Nucleotidylexotransferase (Acute Lymphoblastic Leukemia Marker) Antibody

Specificity: Terminal deoxynucleotidyltransferase (TdT) is a DNA polymerase which catalyzes the addition of deoxyribonucleotides onto the 3'-hydroxyl end of DNA primers without template direction. The enzyme thus provides a unique method for the labeling of the 3' termini of DNA. The human TdT gene maps to chromosome 10q24.1 and encodes a 510 amino acid protein. Human TdT is synthesized as a single chain peptide that elicits a minor preference for incorporation of deoxyribonucleotides over ribonucleotides forming DNA strands. TdT is present in immature thymocytes, some bone marrow cells, transformed pre-B and pre-T cell lines, and leukemia cells.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 52-192) of human DNTT protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: TdT

Background:

Deoxynucleotidyltransferase terminal, DNA Nucleotidylexotransferase, DNTT, Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase, TDT, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal deoxyribonucleotidyltransferase, Terminal transferase

Human thymus. Jurkatcells.

Molecular Weight: 58kDa

Gene ID: 1791, 534206

UniProt: P04053

Pathways: DNA Damage Repair