Recombinant MCM3 antibody (AA 650-750)

Catalog No. ABIN7860551

This Rabbit Monoclonal antibody specifically detects MCM3 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant MCM3 antibody (AA 650-750) (ABIN7860551)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MCM3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MCM3-8972R

Product Details anti-MCM3 Antibody

Binding Specificity: AA 650-750

Purpose: Recombinant MCM3 (Proliferation Marker) Antibody

Specificity: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (aa 650-750) of human MCM3 protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background:

Cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor mcm3, HCC5, hRlf beta subunit, Human cervical cancer proto oncogene 5, MGC1157, Minichromosome maintenance complex component 3, Minichromosome maintenance deficient 3, P102 protein, Replication licensing factor beta subunit, RLF beta subunit, RLF subunit beta

MCF-7, Jurkat or Ramos cells. Human colon or tonsil.

Molecular Weight: 115kDa

Gene ID: 4172, 179565

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA