Actin antibody

Catalog No. ABIN7863314

This Mouse Monoclonal antibody specifically detects Actin in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse, Rat, Guinea Pig and Hamster.

Quick Overview for Actin antibody (ABIN7863314)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ACTA1-360

Product Details anti-Actin Antibody

Purpose: Actin alpha 1, Skeletal Muscle Antibody

Specificity: All eukaryotic cells express Actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human ACTA1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Actin alpha 1, Skeletal Muscle

Background:

a actin, ACTA, ACTA1, Actin, alpha skeletal muscle, ACTS_HUMAN, Alpha actin 1, Alpha-actin-1, ASMA, MPFD, Skeletal muscle alpha actin 1

Hela or T47D cells, Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Human skeletal muscle, Mouse skeletal muscle, Rat skeletal muscle, Hamster skeletal muscle or Guinea pig skeletal muscle

Molecular Weight: 43kDa

Gene ID: 58, 1288

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development