Recombinant Actin antibody

Catalog No. ABIN7860841

The Rabbit Monoclonal anti-Actin antibody (Clone ACTA1-9105R) (ABIN7860841) specifically detects Actin in WB, IHC, IF and FACS. The antibody is reactive with Human, Mouse, Rat, Guinea Pig and Hamster samples.

Quick Overview for Recombinant Actin antibody (ABIN7860841)

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Guinea Pig, Hamster

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ACTA1-9105R

Product Details anti-Actin Antibody

Purpose: Recombinant Actin alpha 1, Skeletal Muscle Antibody

Specificity: All eukaryotic cells express Actin, which often constitutes as much as 50 % of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas b and g are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human ACTA1 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: Actin alpha 1, Skeletal Muscle

Background:

a actin, ACTA, ACTA1, Actin, alpha skeletal muscle, ACTS_HUMAN, Alpha actin 1, Alpha-actin-1, ASMA, MPFD, Skeletal muscle alpha actin 1

Human Intestine, Mouse Intestine, Rat Intestine, Hamster Intestine, Guinea pig Intestine, Human skeletal muscle, Mouse skeletal muscle, Rat skeletal muscle, Hamster skeletal muscle or Guinea pig skeletal muscle

Molecular Weight: 43kDa

Gene ID: 58, 1288

UniProt: P68133

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development