Recombinant beta Catenin antibody

Catalog No. ABIN7862677

The Mouse Monoclonal anti-beta Catenin antibody is suitable to detect beta Catenin in samples from Human, Mouse and Rat. It has been validated for IHC, FACS and ELISA.

Quick Overview for Recombinant beta Catenin antibody (ABIN7862677)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Clone: RCTNNB1-1507

Product Details anti-beta Catenin Antibody

Purpose: Recombinant Catenin, beta (p120) Antibody

Specificity: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human beta-Catenin (p120) protein fragment

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: Catenin, beta

Background:

Cadherin associated protein, beta 1 88 kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1

HeLa or MCF-7 cells. Liver tissue lysate.

Molecular Weight: 92kDa

Gene ID: 1499, 476018

UniProt: P35222

Pathways: Peptide Hormone Metabolism