Recombinant CKM antibody

Catalog No. ABIN7861140

The Rabbit Monoclonal anti-CKM antibody is suitable to detect CKM in samples from Human. It has been validated for ELISA.

Quick Overview for Recombinant CKM antibody (ABIN7861140)

Target: CKM (Creatine Kinase, Muscle (CKM))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CKM antibody is un-conjugated

Application: ELISA

Clone: CKMM-13033R

Product Details anti-CKM Antibody

Purpose: Recombinant Creatine Kinase MM (Biomarker for Muscular Injury &, Cardiac Damage) Antibody

Specificity: Creatine kinases (CKs) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, electrocytes, retina photoreceptor cells, brain cells, kidney, salt glands, myometrium, placenta, pancreas, thymus, thyroid, intestinal epithelial cells, endothelial cells, cartilage and bone cells, macrophages, blood platelets, and tumor and cancer cells. Human cytoplasmic creatine kinase-B, also designated CK-B and BCK, is a 381 amino acid, brain tissue-specific isoform of creatine kinase. Human cytoplasmic creatine kinase-M (CK-M, MCK) is a muscle tissue specific isoform of creatine kinase. Human cytoplasmic creatine kinase-Mi (Mi-CK, MtCK) is a 416 amino acid mitochondrial-specific isoform of creatine kinase. Cytosolic creatine kinases are important in the energetic regulation of Ca2+-pumps and in the maintenance of Ca2+-homeostasis. The antibody binds specifically to the human creatine-kinase-MM subtype (and not the MB or BB subtype). This antibody binds to human muscle creatine-kinase-MM, an enzyme that catalyzes the conversion of creatine and ATP to phosphocreatine and ADP, generating an ATP-buffer in the muscle. The antibody blocks CK-MM activity by 80 % .

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CKM protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CKM

Target: CKM (Creatine Kinase, Muscle (CKM))

Alternative Name: Creatine Kinase MM

Background:

CKM, CKMM, Creatine kinase M, Creatine kinase M chain, Creatine kinase M type, Creatine kinase M-type, Creatine kinase muscle, Creatine kinase, muscle type, M-CK, MCK, Muscle creatine kinase

Human heart tissue. Skeletal muscle lysate.

Molecular Weight: 43kDa

Gene ID: 1158, 334347

UniProt: P06732