Recombinant CTLA4 antibody

Catalog No. ABIN7861192

The Mouse Monoclonal anti-CTLA4 antibody (Clone rCTLA4-7219) (ABIN7861192) specifically detects CTLA4 in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant CTLA4 antibody (ABIN7861192)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTLA4 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RCTLA4-7219

Product Details anti-CTLA4 Antibody

Purpose: Recombinant CTLA4 / CD152 (Negative Regulator of T-Cells) Antibody

Specificity: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is a member of the CD28 superfamily and is a negative regulator of T cell-mediated immune responses. CTLA-4 expression is induced on the surface of T cells after CD28 binding and activation, and is constitutively expressed on T-regulatory cells, acting as an immune checkpoint inhibitor, downregulating T cell activity. CTLA-4 primarily inactivates T-cell activity by competing with the CD28 costimulatory molecule. CD28 and CTLA-4 share the identical ligands of CD80 and CD86 on antigenpresenting cells, and thus CTLA-4 competes with CD28 function in T-cell survival, proliferation, and recruitment. In particular, CTLA-4 down-modulates CD4+ helper T-cell activity and enhances Treg immunosuppressive functions. CTLA-4 has been shown to play a role in human diseases. CTLA-4 acts as a physiological brake on the activated immune system in order to maintain immune homeostasis. Several suppressive mechanisms for T-cell functions have been attributed to CTLA-4.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human CTLA4 (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CTLA4

Background:

ALPS5, CD152, Celiac disease 3 (CELIAC3), Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4), GRD4, GSE, ICOS, Insulin-dependent Diabetes Mellitus 12 (IDDM12)

Jurkat or Raji cells. Lymph node or tonsil.

Molecular Weight: 30-43kDa

Gene ID: 1493, 247824

UniProt: P16410

Pathways: Cancer Immune Checkpoints