DNMT3A antibody

Catalog No. ABIN7862712

The Mouse Monoclonal anti-DNMT3A antibody is suitable to detect DNMT3A in samples from Human. It has been validated for WB and IHC.

Quick Overview for DNMT3A antibody (ABIN7862712)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DNMT3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: PCRP-DNMT3A-1E2

Product Details anti-DNMT3A Antibody

Purpose: DNMT3A / DNA Methyltransferase 3 Alpha Antibody

Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. DNMT3A is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Sadenosyl-L-methionin

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length DNMT3A protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Alternative Name: DNMT3A

Background:

DNA methyltransferase 3 alpha, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, DNMT, DNMT3A2, M.HsaIIIA, MCMT, TBRS

HeLa cells. Placenta.

Molecular Weight: 102kDa

Gene ID: 1788, 154069

UniProt: Q9Y6K1