DRAP1 antibody

Catalog No. ABIN7861235

This Mouse Monoclonal antibody specifically detects DRAP1 in WB, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for DRAP1 antibody (ABIN7861235)

Target: DRAP1 (DR1-Associated Protein 1 (Negative Cofactor 2 Alpha) (DRAP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DRAP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: PCRP-DRAP1-1A12

Product Details anti-DRAP1 Antibody

Purpose: DRAP1 / NC2 alpha Antibody

Specificity: NC2 (negative cofactor 2) is a dimeric histone-fold complex that represses RNA polymerase II transcription through binding to TBP and inhibiting the transcription factors TFIIA and TFIIB. NC2 consists of two subunits, termed NC2a and NC2≤, and these subunits dimerize and bind to TBP-promoter complexes via histone fold domains of the H2A-H2B type. NC2 associates with promoters in a manner that correlates with transcriptional activity and with occupancy by basal transcription factors. NC2 binds directly to DNA, and the binding of NC2 to TBP-promoter complexes affects the conformation of DNA, and results in the inhibition of TFIIB.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human DRAP1 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for DRAP1

Target: DRAP1 (DR1-Associated Protein 1 (Negative Cofactor 2 Alpha) (DRAP1))

Alternative Name: DRAP1

Background:

DR1 associated corepressor, DR1 associated protein 1 (negative cofactor 2 alpha), DR1 associated protein 1, DRAP 1, Drap1, NC2 alpha, Negative co-factor 2-alpha

HeLa or MCF-7, FADU cells. Human brain|testis or smooth muscle.

Molecular Weight: 22.35Da

Gene ID: 10589, 356742

UniProt: Q14919