E2F6 antibody

Catalog No. ABIN7861239

The Mouse Monoclonal anti-E2F6 antibody is suitable to detect E2F6 in samples from Human. It has been validated for IF and FACS.

Quick Overview for E2F6 antibody (ABIN7861239)

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E2F6 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-E2F6-1F8

Product Details anti-E2F6 Antibody

Purpose: E2F6 Antibody

Specificity: The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an active E2F transcriptional regulatory complex.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human E2F6 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for E2F6

Target: E2F6 (E2F Transcription Factor 6 (E2F6))

Alternative Name: E2F6

Background:

EMA, MGC111545

HeLa or Jurkat cells.

Molecular Weight: 35kDa

Gene ID: 1876, 603093

UniProt: O75461