E4F1 antibody

Catalog No. ABIN7862718

The Mouse Monoclonal anti-E4F1 antibody (Clone PCRP-E4F1-2D1) (ABIN7862718) specifically detects E4F1 in IF and FACS. The antibody is reactive with Human samples.

Quick Overview for E4F1 antibody (ABIN7862718)

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This E4F1 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-E4F1-2D1

Product Details anti-E4F1 Antibody

Purpose: E4F1 Antibody

Specificity: E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control. Additionally, E4F1 is thought to function as a ubiquitin ligase that mediates the ubiquitination (and subsequent degradation) of target proteins and may be involved in the p53 tumor suppressor pathway. E4F1, which may be post-translationally phosphorylated or sumoylated, is subject to proteolytic cleavage which results in the creation of a short peptide with specific DNA binding capabilities.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human E4F1 protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for E4F1

Target: E4F1 (E4F Transcription Factor 1 (E4F1))

Alternative Name: E4F1

Background:

E4F transcription factor 1, isoform CRA_b, P120E4F transcription factor, p50E4F, Putative E3 ubiquitin-protein ligase E4F1, RING type E3 ubiquitin transferase E4F1, Transcription factor E4F1

HeLa, Jurkat or MCF7 cells.

Molecular Weight: 83kDa

Gene ID: 1877, 513268

UniProt: Q66K89