EIF4A2 antibody

Catalog No. ABIN7862734

The Mouse Monoclonal anti-EIF4A2 antibody (Clone PCRP-EIF4A2-2B5) (ABIN7862734) specifically detects EIF4A2 in ELISA. The antibody is reactive with Human samples.

Quick Overview for EIF4A2 antibody (ABIN7862734)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF4A2 antibody is un-conjugated

Application: ELISA

Clone: PCRP-EIF4A2-2B5

Product Details anti-EIF4A2 Antibody

Purpose: EIF4A2 (Eukaryotic Initiation Factor 4A-II) Antibody

Specificity: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5'-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5' cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human EIF4A2 protein

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: EIF4A2

Background:

ATP dependent RNA helicase eIF4A 2, BM-010, DDX2B, EIF4F, eukaryotic translation initiation factor 4A isoform 2, N-terminally processed

HeLa cells.

Molecular Weight: 46kDa

Gene ID: 1974

UniProt: Q14240