Recombinant Fascin antibody

Catalog No. ABIN7863397

The Mouse Monoclonal anti-Fascin antibody is suitable to detect Fascin in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant Fascin antibody (ABIN7863397)

Target: Fascin (FSCN1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fascin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RFSCN1-6464

Product Details anti-Fascin Antibody

Purpose: Recombinant Fascin-1 (Reed-Sternberg CellMarker) Antibody

Specificity: Recognizes a protein of 55 kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin s disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Full length recombinant human FSCN1 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Fascin

Target: Fascin (FSCN1)

Alternative Name: Fascin-1

Background:

55 kDa actin-bundling protein, FAN1, Fascin homolog 1, Fascin1, FSCN1, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like), SNL

HeLa, MCF-7, Ovarian or Testicular Carcinoma., PC-3 or BEWO cells. Hodgkin s lymphoma

Molecular Weight: 55kDa

Gene ID: 6624, 118400

UniProt: Q16658