Geminin antibody

Catalog No. ABIN7863210

The Mouse Monoclonal anti-Geminin antibody is suitable to detect Geminin in samples from Human. It has been validated for IHC and IF.

Quick Overview for Geminin antibody (ABIN7863210)

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Geminin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: GMNN-3665

Product Details anti-Geminin Antibody

Purpose: Geminin / DNA Replication Inhibitor Antibody

Specificity: Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human GMNN protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Geminin

Target: Geminin (GMNN) (Geminin, DNA Replication Inhibitor (GMNN))

Alternative Name: Geminin

Background:

DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GMNN, RP3 369A17.3

HEK293, HeLa or MCF-7 cells. Human tonsil, testis or triple-negative breast cancer tissue.

Molecular Weight: 35kDa

Gene ID: 51053, 234896

UniProt: O75496

Pathways: EGFR Signaling Pathway, DNA Replication