GTF2H2 antibody

Catalog No. ABIN7862927

The Mouse Monoclonal anti-GTF2H2 antibody is suitable to detect GTF2H2 in samples from Human. It has been validated for IF and FACS.

Quick Overview for GTF2H2 antibody (ABIN7862927)

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GTF2H2 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-GTF2H2-1B9

Product Details anti-GTF2H2 Antibody

Purpose: GTF2H2 / BTF2 / TFIIH Basal Transcription Factor Antibody

Specificity: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and Mat1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human GTF2H2 protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for GTF2H2

Target: GTF2H2 (General Transcription Factor IIH, Polypeptide 2, 44kDa (GTF2H2))

Alternative Name: GTF2H2

Background:

Basic transcription factor 2 44 kDa subunit, BTF2, BTF2-p44, BTF2P44, General transcription factor IIH, polypeptide 2, 44 kDa, T-BTF2P44, TFIIH basal transcription factor complex p44 subunit

HeLa or MCF7 cells.

Molecular Weight: 44kDa

Gene ID: 2966, 607501

UniProt: Q13888

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling