CD25 antibody

Catalog No. ABIN7863002

This Mouse Monoclonal antibody specifically detects CD25 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD25 antibody (ABIN7863002)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: IL2RA-2393

Product Details anti-CD25 Antibody

Purpose: CD25 / IL2RA (Activated Lymphocyte Marker) Antibody

Specificity: Recognizes a protein of 55 kDa, identified as CD25 (Workshop IV, Code A27). CD25 is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkin's disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 Molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R chain, p75) and CD132 (common chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This MAb recognizes the epitope B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Alternative Name: CD25

Background:

IL2 Receptor alpha, IL2R alpha chain, IL2R, IL2R subunit alpha IL2RA, Interleukin-2 receptor subunit alpha, Ly43, p55, p55 chain, T Cell Growth Factor Receptor, TAC antigen, TCGFR

PHA-stimulated human lymphocytes. Human lymph nodes and tonsils. Ramos, HeLa or Jurkat.

Molecular Weight: 55kDa

Gene ID: 3559, 231367

UniProt: P01589

Pathways: JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation