MITF antibody

Catalog No. ABIN7863138

The Mouse Monoclonal anti-MITF antibody is suitable to detect MITF in samples from Human. It has been validated for WB, IF and FACS.

Quick Overview for MITF antibody (ABIN7863138)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MITF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: PCRP-MITF-1D9

Product Details anti-MITF Antibody

Purpose: Microphthalmia Transcription Factor (MITF) Antibody

Specificity: MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MITF protein

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Alternative Name: Microphthalmia Transcription Factor

Background:

BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A

A-431, HeLa or 501 Mel human melanoma cells.Human melanoma., Jurkat

Molecular Weight: 52-56kDa (doublet)

Gene ID: 4286

UniProt: O75030

Pathways: Chromatin Binding