Nidogen antibody

Catalog No. ABIN7861825

The Rat Monoclonal anti-Nidogen antibody (Clone ELM1) (ABIN7861825) specifically detects Nidogen in ELISA. The antibody is reactive with Mouse and Guinea Pig samples.

Quick Overview for Nidogen antibody (ABIN7861825)

Target: Nidogen (NID)

Reactivity: Mouse, Guinea Pig

Host: Rat

Clonality: Monoclonal

Conjugate: This Nidogen antibody is un-conjugated

Application: ELISA

Clone: ELM1

Product Details anti-Nidogen Antibody

Purpose: Nidogen / Entactin Antibody

Specificity: It reacts with the intact (150 kDa) nidogen as well as its proteolytic fragments of 135 kDa, 110 kDa, 100 kDa, and 80 kDa. This MAb does not cross-react with other basement membrane components or fibronectin. Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Murine EHS laminin preparation

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Nidogen

Target: Nidogen (NID)

Alternative Name: Nidogen

Background:

Nid1, Ent, Entactin 1, NID-1, Nidogen-1,

Mouse kidney or eye tissue.

Molecular Weight: 100|105|110|135|and 80kDa