Nidogen antibody

Catalog No. ABIN7881810

This Rat Monoclonal antibody specifically detects Nidogen in ELISA. It exhibits reactivity toward Mouse.

Quick Overview for Nidogen antibody (ABIN7881810)

Target: Nidogen (NID)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Nidogen antibody is un-conjugated

Application: ELISA

Clone: ELM1

Product Details anti-Nidogen Antibody

Purpose: Nidogen Antibody

Purification: Protein G affinity chromatography

Immunogen: A murine EHS laminin preparation was used as the immunogen for the Nidogen antibody.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal dilution of the Nidogen antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Nidogen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for Nidogen

Target: Nidogen (NID)

Alternative Name: Nidogen

Background: Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation. This MAb does not cross-react with other basement membrane components or fibronectin.

UniProt: P10493