POLE3 antibody

Catalog No. ABIN7863249

This Mouse Monoclonal antibody specifically detects POLE3 in IF, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for POLE3 antibody (ABIN7863249)

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This POLE3 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: PCRP-POLE3-2F10

Product Details anti-POLE3 Antibody

Purpose: POLE3 / CHRAC17 Antibody

Specificity: DNA replication is initiated by the binding of initiation factors to the origin of replication. Nucleosomes inhibit access to the replication machinery at these origin sequences. Nucleosome remodeling factors increase the accessibility of nucleosomal DNA to transcriptional regulators. CHRAC15 and CHRAC17 are subunits of the nucleosomal remodeling factor CHRAC (chromatin accessibility complex), which increases the accessibility of nucleosomal DNA in an ATP-dependent manner. Unlike other known chromatin remodeling factors, CHRAC also functions during chromatin assembly by using ATP to convert irregular chromatin into a regular array of nucleosomes with even spacing. This conversion process occurs when CHRAC organizes randomly deposited histones into a regularly spaced array. In the presence of CHRAC, the nucleosomal ATPase ISWI catalyzes several ATP-dependent transitions of chromatin structure.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human POLE3 protein

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for POLE3

Target: POLE3 (Polymerase (DNA Directed), epsilon 3 (p17 Subunit) (POLE3))

Alternative Name: POLE3

Background:

Arsenic transactivated protein, ASTP, CHRAC17, Chromatin accessibility complex 17 kDa protein, DNA polymerase II subunit 3, Histone fold protein CHRAC17, HuCHRAC17, Polymerase (DNA directed) epsilon 3 (POLE3), YBL1

HeLa or K562 cells.

Molecular Weight: 16.86kDa

Gene ID: 54107, 108112

UniProt: Q9NRF9

Pathways: DNA Damage Repair