Recombinant NeuN antibody

Catalog No. ABIN7862669

The Rabbit Monoclonal anti-NeuN antibody (Clone NeuN-288R) (ABIN7862669) specifically detects NeuN in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for Recombinant NeuN antibody (ABIN7862669)

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This NeuN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: NeuN-288R

Product Details anti-NeuN Antibody

Purpose: Recombinant Neuronal-Nuclei (NeuN) (Neuronal Marker) Antibody

Specificity: NeuN antibody specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuro. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function, however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human NeuN protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for NeuN

Target: NeuN (RBFOX3) (RNA Binding Protein, Fox-1 Homolog 3 (RBFOX3))

Alternative Name: Neuronal-Nuclei

Background:

FLJ56884, FLJ58356, fox1 homolog C, Fox3, FOX3NeuN, hexaribonucleotide binding protein 3 (HRNBP3), neuronal nuclei, Rbfox3, RNA binding protein, fox 1 homolog (C. elegans) 3

Human brain. HeLa cells.

Molecular Weight: 48kDa

Gene ID: 146713, 135229

UniProt: A6NFN3