ROR gamma antibody

Catalog No. ABIN7863353

This Mouse Monoclonal antibody specifically detects ROR gamma in IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for ROR gamma antibody (ABIN7863353)

Target: ROR gamma

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: RORC-2941

Product Details

Purpose: ROR-gamma / RORC (RAR-related Orphan Receptor C) Antibody

Specificity: This MAb recognizes a protein of 63 kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors ROR°™ and RORÁ are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50 % amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered orphan receptors, due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human RORC protein

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: ROR gamma

Alternative Name: ROR-gamma

Background:

Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3 (NR1F3), RAR related orphan receptor C, Retinoid-related orphan receptor-gamma (RORG), Rorc, RZR GAMMA, RZRG, TOR

MOLT4 cells. Human lymphocytes. Human liver or skeletal muscle tissue.

Molecular Weight: 63kDa

Gene ID: 6097

UniProt: P51449