Recombinant Transglutaminase 2 antibody

Catalog No. ABIN7863450

This Rabbit Monoclonal antibody specifically detects Transglutaminase 2 in WB, IHC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Transglutaminase 2 antibody (ABIN7863450)

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Transglutaminase 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: TGM2-6943R

Product Details anti-Transglutaminase 2 Antibody

Purpose: Recombinant Transglutaminase II (TGM2) (FN Binding Domain) Antibody

Specificity: Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues. Extracellular tTG is able to bind to and cross-link several ECM proteins and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation. High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human TGM2 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Transglutaminase 2

Target: Transglutaminase 2 (TGM2) (Transglutaminase 2 (C Polypeptide, Protein-Glutamine-gamma-Glutamyltransferase) (TGM2))

Alternative Name: Transglutaminase II

Background:

Protein-glutamine gamma-glutamyltransferase 2, TG(C), TG2, TGase C, TGase H, TGase-2, TgaseII, TGC, TGM2, Tissue type transglutaminase, Transglutaminase 2 C polypeptide, Transglutaminase H, tTG, tTGas

Brain, HUVEC cells. Endothelial cells in placenta, Liver, or breast carcinoma. Smooth muscle cells of any origin.

Molecular Weight: 77-85kDa

Gene ID: 7052, 517033

UniProt: P21980

Pathways: Tube Formation, Thromboxane A2 Receptor Signaling