Vinculin antibody

Catalog No. ABIN7863493

The Mouse Monoclonal anti-Vinculin antibody (Clone VCL-3617) (ABIN7863493) specifically detects Vinculin in IHC, IF and FACS. The antibody is reactive with Human, Mouse, Rat, Rabbit, Pig, Cow, Avian, Fish and Frog samples.

Quick Overview for Vinculin antibody (ABIN7863493)

Target: Vinculin (VCL)

Reactivity: Human, Mouse, Rat, Rabbit, Pig, Cow, Avian, Fish, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vinculin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: VCL-3617

Product Details anti-Vinculin Antibody

Purpose: Vinculin (Marker of Age-related Macular Degeneration) Antibody

Specificity: This antibody recognizes both Vinculin (125 kDa) and meta-Vinculin (135 kDa). Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and °™-Actinin. In addition, vinculin, Talin and °™-Actinin each contain Actin binding sites. Expression of vinculin and Talin have been shown to be affected by the level of Actin expression. °™-Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Vinculin protein (VCL)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vinculin

Target: Vinculin (VCL)

Alternative Name: Vinculin

Background:

CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, VCL, VINC

A431, K562, NIH3T3, THP-1 cells. Bladder and Testis, U-87

Molecular Weight: 125-135kDa

Gene ID: 7414, 643896

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2