MCM3 antibody (AA 650-750)

Catalog No. ABIN7865520

The Mouse Monoclonal anti-MCM3 antibody (Clone MSVA-503M) (ABIN7865520) specifically detects MCM3 in IHC. The antibody is reactive with Human samples.

Quick Overview for MCM3 antibody (AA 650-750) (ABIN7865520)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM3 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-503M

Product Details anti-MCM3 Antibody

Binding Specificity: AA 650-750

Purpose: MCM3 (Proliferation Marker) Antibody

Specificity: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Purification: Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (aa 650-750) of human MCM3 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background:

Cervical cancer proto-oncogene 5, DNA polymerase alpha holoenzyme-associated protein P1, DNA replication factor MCM3, DNA replication licensing factor mcm3, HCC5, hRlf beta subunit, Human cervical cancer proto oncogene 5, MGC1157, Minichromosome maintenance complex component 3, Minichromosome maintenance deficient 3, P102 protein, Replication licensing factor beta subunit, RLF beta subunit, RLF subunit beta

Colon: A strong nuclear MCM3 immunostaining should be seen in virtually all crypt base cells.

Molecular Weight: 115kDa

Gene ID: 4172, 179565

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA